Quantification of thiols and disulfides
نویسندگان
چکیده
منابع مشابه
synthesis of sulfides from alcohols and thiols in solvent-freeconditions and deoxygenation of sulfoxides
کاتالیست یک سنتز جدید برای تیواترها توصیف شده است. واکنش الکل ها با آریل، هتروآریل و آلکیل تیو ل ها درحضور 1،3،5- تری آزو- 2،4،6- تری فسفرین-2،2،4،4،6،6 هگزاکلراید ((tapc به عنوان یک کاتالیست موُثر، بازده های خوب تا عالی از تیواترها را حاصل می کند. علاوه براین، واکنش تحت شرایط بدون فلز و بدون حلال پیش می رود، بنابراین یک مکمل جالب برای روش های شناخته شده سنتز تیواترها ارائه می دهد. یک مکانیسم ا...
15 صفحه اولQuantification of Global Protein Disulfides and Thiol-Protein Mixed Disulfides to Study the Protein Dethiolation Mechanisms
The redox state of cellular thiols is widely studied because it was recently linked to many different diseases and pathologies. In this work we quantified the concentrations of protein disulfides (PSSP) and thiol-protein mixed disulfides (XSSP) in rat tissues (liver, kidney and heart) and cells (Raw 264.7) by an improved method of XSSP and PSSP determination after oxidative stress induced by di...
متن کاملMicrowave assisted oxidation coupling of thiols to symmetrical disulfides with tripropylammonium fluorochromate (VI) (TPAFC)
Tripropylammonium fluorochromate(VI) (TPAFC), is an efficient and new reagent, which isprepared easily and oxidizes thiols to the corresponding disulfides, quickly. The reactions areperformed cleanly and are controlled to stop at the disulfide stage, without over-oxidation andside products. Coupling of thiols to their corresponding disulfides, are studied in solution atroom temperature and in s...
متن کاملThe effect of thiols and disulfides on water uptake and extrusion by rat liver mitochondria.
Reduced glutathione is among the substances of physiological occurrence which stimulate water uptake by isolated rat liver mitochondria (l-3). The water uptake produced by glutathione is of special interest since it differs in some respects from that induced by other agents such as phosphate, thyroxine, and phlorizin (l-6). Perhaps the most significant difference is that glutathione-swollen mit...
متن کاملThe role of thiols and disulfides in protein chemical and physical stability
The use of proteins for therapeutic applications has increased dramatically in the last several decades. There has been a tremendous increase in the number of approved drugs derived from recombinant proteins and monoclonal antibodies since the approval of recombinant insulin in 1982 (Figure 1).1 In addition, the number of approved indications is almost four times the number of approved drugs in...
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ژورنال
عنوان ژورنال: Biochimica et Biophysica Acta (BBA) - General Subjects
سال: 2014
ISSN: 0304-4165
DOI: 10.1016/j.bbagen.2013.03.031